Speakers -------- N/A Successful engineering and designing of vectors and clone constructs leads to efficient production throughout the protein expression pipeline. Protein expression bottlenecks frequently arise because functional proteins are difficult to produce as they fail to express, are expressed as insoluble aggregates, or cannot be purified by standard methods. Thus, protein engineers are forced to return to the drawing board. This usually requires designing new cloning schemes including: lengthy verification and sequence analysis of the gene or protein of interest, moving a gene from one vector to another, transfecting the vector in an alternative host, re-characterizing the expressed protein, or any or all of the above. Researchers view this process as a linear pathway: make an expression clone, try it out, and if it fails, go back to the beginning and start over--an inefficient, time-consuming and expensive process. Cambridge Healthtech Instituteâ€™s Inaugural Engineering Vectors and Designing Clone Constructs continues the tradition of applying protein discovery research leading to functional products. Learn from seasoned savvy researchers as they share their real-world experiences, applications, and results.
|Thursday, January 14, 2010|
|Friday, January 15, 2010|