Biotech China 2014
Difficult to Express Proteins
Sheraton Boston Hotel

Boston, Massachusetts
http://www.pegsummit.com/dpx
   Speakers
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   N/A

   Membrane proteins, ion channels, toxins, vaccines and other “finicky”
   proteins often are the best choices for therapeutics, yet their
   successful expression is rife with roadblocks and challenges. This
   conference will present innovative and imaginative methods to make
   those difficult to express proteins more cooperative.

Schedule of Presentations:

Monday, May 17, 2010
07:00:00 Registration and Morning Coffee
08:25:00 IMPROVING PERFORMANCE FROM THE GROUND UP
08:30:00 Chairperson’s Opening Remarks
08:40:00 KEYNOTE PRESENTATION - Breaking the Cell Wall Barrier for Difficult-to-Produce Natural and Supernatural Products James R. Swartz
09:10:00 Screening Approaches to Solving Expression and Solubility Problems Using Novel Tagging and Detection System Geoffrey Waldo
09:40:00 Applications and Expansions of Expression Modules That Tag Mammalian Membrane Proteins Li Lin
10:10:00 Grand Opening Coffee Break in Exhibit Hall
11:10:00 Evaluating Host Cell Differences for Difficult to Generate Proteins Jennitte Stevens
11:40:00 Meeting the Gene Expression Challenges Posed by Heterologous Polyketide Biosynthesis Blaine A. Pfeifer
12:10:00 Dual Purpose Aminoacyl-tRNA Synthetases A. James Link
12:40:00 Presentation Chris Finnis
12:55:00 Luncheon Presentation I - Human In Vitro Translation Systems for Rapid, High Fidelity Protein Production Brian Webb
13:10:00 Luncheon Presentation II (Sponsorship Opportunities Available) or Lunch on Your Own
13:55:00 EXPRESSING PROTEINS FOR STRUCTURE
14:00:00 Chairperson’s Remarks Philip Laible
14:05:00 Assessment of the Importance of Sample Purity on Membrane-Protein Crystallization in Different Systems Philip Laible
14:35:00 Expression, Refolding and Purification of a Human IL-17A Variant for Structural Studies Bingyuan Wu
15:05:00 Refreshment Break, Poster and Exhibit Viewin
15:40:00 GLYCOSYLATION
15:45:00 Robust, Automated, High-Throughput Quantitative HPLC-Based Platform for Glycan Analysis with Computer-Assisted Data Interpretation Pauline Rudd
16:15:00 Engineering N-Glycosylation in the Baculovirus Expression System Christoph Geisler
16:45:00 Problem Solving Break-Out Sessions
16:50:00 Enhancing Cytoplasmic Expression in Mammalian Cells Dominic Esposito
17:00:00 Getting a Handle on Proteins Using the Right Tag Geoffrey Waldo
17:15:00 Mammalian membrane proteins: how to break the bottleneck Li Lin
17:30:00 Novel Analytical Techniques/Tools for the Characterization of Biopharmaceuticals Jennifer F. Nemeth
17:45:00 Networking Cocktail Reception in the Exhibit Hall
Tuesday, May 18, 2010
08:20:00 EXPRESSING “FINICKY PROTEINS:” CASE STUDIES IN SUCCESS
08:25:00 Chairperson’s Opening Remarks Jean-Luc Lenormand
08:30:00 HaloTag® Based Purification of Functional Proteins from Mammalian Cells Rachel Friedman Ohana
09:00:00 Employing Rhodobacter sphaeroides to Functionally Express and Purify Human G Protein-Coupled Receptors Ankita Roy
09:30:00 Comparison of High-Throughput Techniques for the Expression of Protein Complexes Gyorgy Babnigg
10:00:00 Coffee Break, Poster and Exhibit Viewing
10:45:00 Production of Recombinant Proteoliposomes for Therapeutic Uses Jean-Luc Lenormand
11:15:00 Co-Expression of AMPK Subunits in Insect Cells Yields a Stable Heterotrimer Lata Ramanathan
11:45:00 Elimination of Bacterial Toxicity of the Gene Encoding the Tfg1 Subunit of S. cerevisiae TFIIF Alfred S. Ponticelli
12:15:00 Expression of a Vaccine Antigen Candidate Protein in the Pseudomonas Fluorescens-Based Pfenex Expression Technology Bruce Carpick
12:45:00 Luncheon Presentation (Sponsorship Opportunities Available) or Lunch on Your Own
13:55:00 UNIQUE HOSTS AND TOOLS
14:00:00 Chairperson’s Remarks Dominic Esposito
14:05:00 Expression Plasmids and Production of EGFP in Stably Transfected Acanthamoeba Erik A. Bateman
14:35:00 The Science of Solubility: Structure/Function Studies of Chimeric NusA Fusion Tags Provides Insight into Protein Solubility Enhancement Dominic Esposito
15:05:00 Functional Assembly of Membrane-Associated Protein Complexes on a Derivatized Lipid Template for HTS – a SmartScreen™ Technology Review Scott Gridley
15:20:00 Advancing Synthetic Gene Design Mark Welch
15:35:00 Refreshment Break, Poster and Exhibit Viewing
16:15:00 A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells Eric Campeau
16:45:00 Development of Algal Chloroplasts as a Novel Bioreactor for the Production of Pharmaceutical Proteins Shengwu Ma
17:15:00 End of Conference
4th Annual Pharma R&D Asia Congress
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