Biotech China 2014

Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells

TypeAbstract Summary

Transcription factors (TFs) and their specific interactions with targets are crucial for specifying gene-expression programs. To gain insights into the transcriptional regulatory networks in embryonic stem (ES) cells, we use chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing (ChIP-seq) to map the locations of 13 sequence-specific TFs (Nanog, Oct4, STAT3, Smad1, Sox2, Zfx, c-Myc, n-Myc, Klf4, Esrrb, Tcfcp2l1, E2f1, and CTCF) and 2 transcription regulators (p300 and Suz12). These factors are known to play different roles in ES-cell biology as components of the LIF and BMP signaling pathways, self-renewal regulators, and key reprogramming factors. Our study provides insights into the integration of the signaling pathways into the ES-cell-specific transcription circuitries. Intriguingly, we find specific genomic regions extensively targeted by different TFs. Collectively, the comprehensive mapping of TF-binding sites identifies important features of the transcriptional regulatory networks that define ES-cell identity.

Keywords
Authors
Name:Robert Phair
Name:Daniel S Peeper
Name:Wei-Wei Zhang
Name:R S Polishchuk
Name:Chia-Lin Wei
Name:Alexey Khodjakov
Name:Renata Basto
Name:Jeffrey Axelrod
Name:Huck Hui Ng
Name:Jesús Gil
Name:Andreas Bracher
Name:F Ulrich Hartl
Name:Gordon C Shore
Name:Catriona Y Logan
Name:Roel Nusse­
Organizations
Organization:Stanford University

Previous Comments

4th Annual Pharma R&D Asia Congress
About Us | Privacy Policy | Site Map | FAQs | Advertise With Us | Community
BiotechScienceNews.com promotes research and ranks life scientists working in the life science spectrum involving biotechnology,
drug discovery, genomics, microbiology, neuroscience, medicine, pharmacology, cell biology, and molecular biology.