3D Tissue Models
Engineering Vectors and Designing Clone Constructs
Hotel Del Coronado

San Diego, California


   Successful engineering and designing of vectors and clone constructs
   leads to efficient production throughout the protein expression
   pipeline. Protein expression bottlenecks frequently arise because
   functional proteins are difficult to produce as they fail to express,
   are expressed as insoluble aggregates, or cannot be purified by
   standard methods. Thus, protein engineers are forced to return to the
   drawing board. This usually requires designing new cloning schemes
   including: lengthy verification and sequence analysis of the gene or
   protein of interest, moving a gene from one vector to another,
   transfecting the vector in an alternative host, re-characterizing the
   expressed protein, or any or all of the above. Researchers view this
   process as a linear pathway: make an expression clone, try it out, and
   if it fails, go back to the beginning and start over--an inefficient,
   time-consuming and expensive process. Cambridge Healthtech Institute’s
   Inaugural Engineering Vectors and Designing Clone Constructs continues
   the tradition of applying protein discovery research leading to
   functional products. Learn from seasoned savvy researchers as they
   share their real-world experiences, applications, and results.

Schedule of Presentations:

Thursday, January 14, 2010
Friday, January 15, 2010
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