3D Tissue Models
Mechanism and Regulation of Prokaryotic Transcription
Vermont Academy, Saxtons River, Vermont
20 Pleasant St, Saxtons River, 05154, USA
mm, VT
1. RNA polymerase structure and function
2. Initiation and Escape I
3. Initiation and Escape II
4. Transcription elongation and termination
5. Sigma factors and anti-sigma factors
6. Interface of transcription with other cellular 
7. Stress Responses
8. Transcription Regulation
9. Bacterial nucleoid organisation

Schedule of Presentations:

Saturday, June 23, 2007
16:00:00 Conference Registration
18:00:00 FASEB Opening Reception
19:00:00 Dinner
20:30:00 “Small molecules: big effects on transcription”
21:15:00 “Sigma 38 transcription: characteristics and comparisons” Jay D Gralla
Sunday, June 24, 2007
07:30:00 Breakfast
09:00:00 “Molecular bioimaging of gene transcription” Patrick Cramer
09:30:00 “X-ray crystal structure of RNA polymerase from Archaea” Katsuhiko S Murakami
10:00:00 “Structural basis for substrate loading in bacterial RNA polymerase” Dmitri G Vassylyev
10:30:00 Group photo and FASEB-sponsored Coffee Break
11:15:00 “Molecular evolution of multi-subunit RNA polymerases” William Lane
11:30:00 “Mechanism of inhibition of RNA polymerase by rifampicin Andrey Feklistov
11:45:00 “Myxopyronin, corallopyronin, and ripostatin inhibit transcription by binding in the RNA polymerase 'switch region'" Jayanta Mukhopadhyay
12:00:00 Michael Gleghorn, Pennsylvania State University “Structural and functional basis for bacteriophage N4 minivRNAP promoter recognition”
12:30:00 Lunch
16:00:00 Poster Session 1
18:00:00 Dinner
19:00:00 “Roles of upstream and downstream DNA in open complex formation ” Ruth M Saecker
19:30:00 “Time-resolved footprinting of transcription initiation complexes” Bianca Sclavi
20:00:00 “Single molecule analysis of transcription” Richard Ebright
20:30:00 Break
21:00:00 “Real-time detection of the full transcription cycle by singlemolecule DNA nanomanipulation” Terence Strick
21:30:00 “Structural mechanism for the cascade of events that comprise promoter escape by T7 RNAP” Craig T Martin
21:45:00 “Transcription fidelity: parameters that affect sliding and idling of RNAP during elongation” Bill McAllister
Monday, June 25, 2007
07:30:00 Breakfast
09:00:00 “Region 1.1 of group 1 sigma factors” Ed Schwartz
09:30:00 “NusG inhibits sigma action during elongation” Rachel Mooney
09:45:00 “Determining the distribution of E. coli RNAP and associated proteins across transcribed regions in vivo” Joe Wade
10:00:00 “Sigma70 retention in elongation complexes in vivo” Rui Rong Niedfeldt
10:15:00 "Residence of GreB on RNAP and elongation complexes" Harini Ravi
10:30:00 "Modeling rRNA transcription" Stefan Klumpp
10:45:00 FASEB-sponsored Coffee Break
11:15:00 “Transcription activation via sigma 54 activators” Xiaodong Zhang
11:45:00 “Structure/function insights about regulation and function of sigma54-dependent bacterial enhancer binding proteins” Tracy Nixon
12:15:00 Lunch
16:00:00 Poster Session 2
18:00:00 Dinner
19:00:00 “Using force to probe the mechanism of transcription termination” Matthew Larson
19:30:00 “Regulation of transcript elongation and pausing” Robert Landick,
20:00:00 “Molecular mechanism of transcription regulation by RfaH”
20:30:00 Break
21:00:00 "Functional architecture of the T7 RNA polymerase transcription complex and the mechanism of pausing and termination at the T7 concatamer junction" Rui J Sousa
21:15:00 “Lambda Q interacts with the beta flap” Bryce E Nickels
21:30:00 “The phage 82 Q protein antiterminator constructs a NusAdependent shield to the emerging transcript” Jeffrey Roberts
21:45:00 “Archaeal transcription termination” Tom Santangelo
Tuesday, June 26, 2007
07:30:00 Breakfast
09:00:00 “Regulation of heat shock responses” Carol Gross
09:30:00 “The SigR-RsrA redox sensing system of Streptomyces Mark Paget
10:00:00 “Sensing stress: regulation of the extracytoplasmic stress factor, SigmaE” Sarah E Ades
10:30:00 FASEB-sponsored Coffee Break
10:45:00 “Genome-scale location data to reconstruct the transcription regulatory network for E. coli” Bernhard Palsson
11:00:00 “Characterization of a novel sigma factor in Bacillus subtilis” Shawn R MacLellan
11:30:00 “Structural basis of DNA recognition by sigma 54” Michaeleen Callahan Doucleff
12:15:00 Lunch
16:00:00 Poster Session 3
18:00:00 Dinner
19:00:00 “Transcription kinetics in E. coli: what does it mean for a gene to be ‘on’?” Ido Golding
19:30:00 “Mapping the input functions of E coli genes” Anat Bren
20:00:00 “Transcriptional networks in adaptive evolution” Eric M Knight
20:15:00 Break
20:45:00 “Bacterial Rho factor as a tool to monitor RNA polymerase II-dependent transcription in yeast” Rachid Rahmouni
21:15:00 “DNA translocation and transcription-coupled DNA repair” Nigel Savery
21:45:00 “Suppression of backtracking by RNA anchoring” Natalia Komissarova
Wednesday, June 27, 2007
07:30:00 Breakfast
09:00:00 “6S RNA regulation of transcription” Karen Wassarman
09:30:00 “Regulation of sigma 32 by the DnaK-DnaJ chaperone system”
10:00:00 “The role of IscR in sensing Fe-S stress” Patricia J Kiley
10:30:00 “OhrR and oxidative stress” Richard Brennan
10:30:00 FASEB-sponsored Coffee Break
11:00:00 “Control and function of Spx” Peter Zuber
11:45:00 “Regulation of the E. coli mar/sox/rob regulon” Robert G Martin
12:00:00 “RNA polymerase-associated proteins control virulence in Francissella tularensis” Simon Dove
12:15:00 Lunch
16:00:00 Poster Session 4
18:00:00 Dinner
19:30:00 “Cyclic-di-GMP signaling in ‘lifestyle’ switching in E. coli” Regine Hengge,
20:00:00 “Repression by binding of H-NS within a transcription unit” Karin Schnetz
20:30:00 “Regulation of transcription by nitric oxide”
21:00:00 Break
21:30:00 “Regulation of E. coli nucleoid-associated proteins” David Grainger
22:00:00 “The L-rhamnose response of the E. coli transcription activators RhaS and RhaR” Susan M Egan,
22:15:00 “Probing transcription factor dynamics at the single molecule level in a living cell” Gene Wei Li
Thursday, June 28, 2007
07:30:00 Breakfast
09:00:00 “How bacteria coordinate transcription, replication, and chromosome segregation during growth in rich medium” N. Patrick Higgins
09:30:00 “A model for all genomes: the role of transcription” Peter Cook
10:00:00 FASEB-sponsored Coffee Break
10:45:00 “Coupling the distribution of RNA polymerase, global gene expression and chromosome structure in Escherichia coli” Ding J Jin
11:15:00 “Structures of Fis bound to DNA- at last” Reid C Johnson
12:00:00 END OF CONFERENCE (Box Lunches Available)
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