Fourteenth Annual Overcoming Protein Expression Challenges with Solutions
Hotel Del Coronado
1500 Orange Ave.
Coronado, California
Despite decades of research and advances, some areas of protein science remain extremely challenging and complex. Antibodies, vaccines, human proteins, and
other difficult-to-express proteins have fueled new research and expression methodologies and technologies. High-throughput purification and tags promise great
rewards, but still pose important questions for researchers. Cell free expression methods hold great potential, but pose new challenges. This meeting addresses
the newest developments in these protein science fields and provides insights and solutions for conquering these issues.

Schedule of Presentations:

Thursday, January 13, 2011
13:00:00 Conference Registration
13:45:00 Chairperson’s Opening Remarks
13:50:00 Performance by Design: Engineering Functionality into Biopharmaceutical Products Susan Dana Jones
14:20:00 Engineering Type III Secretion in Salmonella Daniel Widmaier
14:50:00 Theory and Application of Solubility-Enhancement Tags in Biomolecular NMR Pei Zhou
15:20:00 Pichia Pastoris Expression System: Taking it to the Limit Roland Weis
15:35:00 Refreshment Break | Exhibit and Poster Viewing
16:30:00 The Use of SUMO-Tag to Improve Protein Expression and Factors Affecting Its Removal Bingyuan Wu
17:00:00 Efficient Tag Combinations for Tandem Affinity Purification
17:30:00 A Rational Design of 5’ UTR for the Optimization of Protein Expression in Prokaryotes Sunjae Lee
18:00:00 Close of Day
Friday, January 14, 2011
07:30:00 Breakfast Presentation
08:00:00 Maximizing Heterologous Protein Expression Through Systematic Gene Design Mark Welch
08:15:00 Chairperson’s Opening Remarks
08:20:00 Expression, Purification and Characterization of Biologically Active Human AMPActivated Protein Kinase (AMPK) Heterodimeric Complexes Francis Rajamohan
08:50:00 Industrial Case Study: Development of an Efficient Purification Process for a Human Growth Factor Recombinantly Expressed in E. coli Inclusion Bodies Shelly A. Pizarro
09:20:00 FATP5 Purification and Activity Doug Wisniewski
09:50:00 Sponsored Presentation
10:05:00 Coffee Break | Exhibit Viewing and Poster Awards
11:00:00 High-Throughput Protein Production: The “Brute Force” Approach Bjørn Voldborg
11:30:00 A Dynamic, Genome-Scale Flux Model of Lactococcus lactis to Increase Specific Recombinant Protein Expression David E. Block
12:00:00 CASE STUDY: Targeted Protein Delivery Using Engineered Virus-Like Particles Deb K. K. Chatterjee
12:30:00 Close of Morning Session
12:45:00 Luncheon Presentation
14:00:00 Chairperson’s Remarks
14:05:00 Plenary Keynote Presentation | High-Speed NMR Analysis of Human Membrane Proteins Expressed by Cell-Free Synthesis Senyon Choe
14:35:00 Detergents in the Purification, Stabilization, and Refolding of Membrane Proteins Dirk Linke
15:05:00 Preparation of Stable Isotope-Labeled GPCR by Bacterial Fermentation Alexei Yeliseev
15:35:00 Scale-Up and Process Development of a Suite of Membrane Bound Proteins (CYP450s) in Saccharomyces cerevisiae Prabuddha Kundu
16:05:00 End of Conference
3D Tissue Models
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