Sixth International Congress on Electron Tomography
EMBL Heidelberg
Im Eichwald 18 69126 Heidelberg, Germany 06221 356-0
Heidelberg, Germany
The conference will bring together researchers working on hardware development, software development, and applications of electron tomography, particularly in biology. It will address breakthroughs in biology derived from tomography studies, as well as recent and upcoming technical developments in electron tomography.

Topics covered will include:


      Cryo-electron tomograms at molecular resolution

      The future of cameras and correctors

      Correlative light and electron microscopy

      Cutting edge image processing algorithms

      High-throughput, large-scale reconstructions of plastic embedded samples

      Sub-tomogram averaging

Schedule of Presentations:

Thursday, May 5, 2011
14:00:00 Arrival and Registration
16:20:00 Welcome
16:30:00 Keynote Session | Electron Tomography | Doing Structural Biology in situ Wolfgang P. Baumeister
17:45:00 Session 1 | Small Cell Cryo-Tomography Grant J Jensen
17:50:00 Deconstructing Cilia and Flagella Function with Cryo-Electron Tomography and Structural Proteomics Daniela Nicastro
18:10:00 Electron tomographic studies of mitosis in picoplankton Lu Gan
18:35:00 Macromolecular organisation of F1Fo ATP synthase in whole mitochondria
18:50:00 Lecture Achilleas Frangakis
19:05:00 Dinner
20:30:00 After Dinner Drinks
Friday, May 6, 2011
09:00:00 Session 2 | Cellular Plastic Tomography Claude Antony
09:05:00 Correlative light microscopy and electron tomography for cell biology Thomas Mueller-Reichert
09:25:00 3D architecture of an epidermal cell fusion event | electron tomography at the tissue level Mikhail Eltsov
09:50:00 New fixation method for cellular cryo-electron microscopy
10:05:00 A killer in a corset: the microtubule cytoskeleton of Trypanosoma brucei
10:20:00 Following the evolution of enterovirus replication organelles in 3D
10:35:00 Coffee Break
11:10:00 Session 3 | Hybrid Methods Andreas Hoenger
11:15:00 Revealing the Molecular Architecture of the Nuclear Pore Complex through Cryo-Electron Tomography Elizabeth Villa
11:40:00 Title to be announced John Briggs
12:05:00 Novel Structures Associated with Microtubules of the Ventral Disc in Giardia intestinalis Cindi Schwartz
12:30:00 A feritin-based label for cellular electron cryo-tomography Qing K. Wang
12:45:00 Lunch
14:30:00 Session 4 | Software Development Achilleas Frangakis
14:30:00 X-ray Cellular Tomography: How the images are formed and how the volumes should be reconstructed
14:55:00 Software Developments for Tomographic Acquisition, Reconstruction, and Analysis David Mastronarde
15:20:00 RAPTOR 2.0 | Alignment of electron tomography datsets with fiducial markers by maximum likelihood Fernando Amat
15:45:00 Automated Segmentation and Computational Analysis of Sub-Cellular Structures in Large-Scale Tomography
16:00:00 Poster Session
18:00:00 Dinner
20:00:00 After Dinner Drinks
Saturday, May 7, 2011
09:00:00 Session 5 | Applications: Large Cell Tomography Ohad Medalia
09:05:00 Cellular Cryo Electron Tomography of Native HIV-1 Budding Sites Kay Grünewald
09:25:00 3D structural analysis of eukaryotic flagella/cilia to reveal their bending mechanism Takashi Ishikawa
09:50:00 Microtubule organization in the final stages of cytokinesis Nadav Elad
10:15:00 3D examination of nuclear architecture reveals coupled dynamics of chromatin and nuclear pores in the malaria parasite Plasmodium falciparum
10:30:00 Coffee Break
11:10:00 Session 6 | Emerging Technologies
11:15:00 The Basal Body Assembly Revealed by Electron Cryo-Tomography Sam Li
11:40:00 Radiation Damage Minimized Electron Tomography of Thick Samples in Cs/Cc-Corrected TEMs
12:05:00 FEI Chief Developer
12:20:00 An Electron Counting Direct Detection Camera for Cryo EM
12:30:00 Lunch
14:15:00 Keynote Session | Cryo-ET Puts Structures into Biological Context Wah Chiu
15:30:00 Session 7 | Various Topics selected from Abstracts Martin Beck
15:35:00 Visualizing the topology of dynamic membrane intermediates in endocytic vesicle budding
15:45:00 Towards hierarchical models for tomogram interpretation
16:00:00 Cryomicroscopy of Weibel-Palade Bodies in Endothelial Cells
16:15:00 Macromolecular Organization of the Chloroplast
16:30:00 Poster Session
18:30:00 Busses to Heidelberg Old Town depart
Sunday, May 8, 2011
09:00:00 Session 8 | Applications: Sub-tomogram averaging John Briggs
09:05:00 Adventures in molecular tomography – extracting molecular conformations from hyper-heterogeneous specimens Kenneth Taylor
09:25:00 Jsubtomo - a new versatile tool for sub-tomogram averaging
09:50:00 The nucleosome organization in native chromatin by cryo-electron tomography Margot Scheffer
10:15:00 Unveiling the structure of membrane proteins in their native environment by contrast transfer function corrected cryo-electron tomography
10:30:00 Structural basis for blocking protein aggregation in Huntington’s disease revealed by cryo-electron tomography
10:45:00 Coffee Break
11:25:00 Session 9 | Future Technologies Sriram Subramaniam
11:30:00 A cellular space odyssee Juergen Plitzko
11:55:00 Focal pair tomography increases potential resolution of cryo electron tomographic data
12:10:00 Pushing the size limit of cryo-electron tomography: the structure of the 42.8 kDA encapsidation signal of MoMuLV
12:25:00 Cryo-electron tomography of Marburg virus particles and their assembly and release from infected cells
12:45:00 Packed Lunch
13:15:00 Busses Depart
3D Tissue Models
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