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Peer Reviewed Papers, Books, Chapters

Year Title Citation Authors Review type Summary Keywords File filename File mime type
1. 2000 In vivo detection of amyloid plaques in a mouse model of Alzheimer's disease, 97[13]: 7609-14
2. 1997 Alpha-synuclein in Lewy bodies, 388[6645]: 839-40, 28
3. 1994 Microtubule stabilizing drugs for the treatment of Alzheimer's disease, Neurobiol. Aging, 15:S87-S89
4. 1991 A68—a major subunit of paired helical filaments and derivatized forms of normal-tau, 251[4994]: 675-8, 8
5. Gene expression is tightly regulated in space and time. Pubmed Gene expression is tightly regulated in space and time. To dissect this process with high temporal resolution, we introduce an optogenetic tool termed blue light-induced chromatin recruitment (BLInCR) that combines rapid and reversible light-dependent recruitment of effector proteins with a real-time readout for transcription. We used BLInCR to control the activity of a cluster of reporter genes in the human osteosarcoma cell line U2OS by reversibly recruiting the viral transactivator VP16. RNA production was detectable ∼2 min after VP16 recruitment and readily decreased when VP16 dissociated from the cluster in the absence of light. Quantitative assessment of the activation process revealed biphasic activation kinetics with a pronounced early phase in cells treated with the histone deacetylase inhibitor SAHA. Comparison with kinetic models of transcription activation suggests that the gene cluster undergoes a maturation process when activated. We anticipate that BLInCR will facilitate the study of transcription dynamics in living cells.This article has an associated First Person interview with the first author of the paper. Histone acetylation; Nuclear organization; Optogenetics; Transcription regulation

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